Raw files, Quality control, Genome Alignment programs, SAM tools and BAM/SAM formats, Variant calling & VCF format, variant analysis and interpretations. Genome Analysis Toolkit (GATK). Transcriptomic data processing, tools and alignment; TopHat2, Differential expression analysis; ht- seq count, DES seq package. Data analysis using online databases; Galaxy, GEO2R,BioJupies.